Tissue cultures are a focus of research in various fields as biology, chemistry, immunology, and medicine. Typically, so-called immortalized cultures are used—these are cell cultures that can multiply indefinitely but are modified in certain ways and therefore differ from cells in actual tissues, such as various cancer cells. Recently, the use of so-called primary cells, which more closely resemble cells in tissues, has been on the rise. There are several sources of these cells, and it is often necessary to differentiate primary cells into a specific cell type.
In biomedicine, osteoblasts (bone cells) are often used in research on bone tissue interactions, such as with implants. Differentiation of primary osteoblasts requires their cultivation in a medium supplemented with specific growth factors. The aim of the internship will be to explore the possibility of differentiating primary osteoblasts using factors covalently immobilized on the surface of the culture vessel, which would simplify and streamline the process of preparing these cells.