Obor: Biophysics

Optimizing DNA-PAINT for Super-Resolution Imaging of Whole Cells

The recent development of super-resolution imaging methods has transformed the role played by fluorescence microscopes in biology. These techniques allow researchers to overcome the classical diffraction limit of light. Recent advancements in points accumulation for imaging in nanoscale topography (PAINT) technology allow to utilize conventional confocal platform to achieve up to 20 nm planar and 80 nm…

Cell Plasticity Under Physical Constraints in 3D Cultures of Hepatic Cell Lines

Despite technical advances and numerous experimental evidence, only recently became widely accepted the concept, that mechanical cues and properties of culture environment play crucial role in modulating cell behaviors. However, molecular foundations, that mediate microenvironment stiffness-induced development of liver pathologies are still not fully understood. Intern will learn following techniques and methods: Standard cell culturing…