Optimizing DNA-PAINT for Super-Resolution Imaging of Whole Cells

The recent development of super-resolution imaging methods has transformed the role played by fluorescence microscopes in biology. These techniques allow researchers to overcome the classical diffraction limit of light. Recent advancements in points accumulation for imaging in nanoscale topography (PAINT) technology allow to utilize conventional confocal platform to achieve up to 20 nm planar and 80 nm axial resolution.

Intern will learn following techniques and methods:

  • Standard cell culturing;
  • Advanced super-resolution cell imaging (DNA-PAINT with spinning disk microscopy);
  • Different microscopy techniques (confocal, spinning disk, epi-fluorescent).

Goal during internship: to optimize DNA-PAINT imaging of different sub-cellular structures (mitochondria, lysosomes, endoplasmic reticulum).